The Montessori School in southwest Reading will be closing at the end of the semester.
Alvernia University has operated the school for the past few years.
Officials said the school will be closing because of rising costs, declining enrollment and an increase in day cares in the area.
The school was founded by the Bernardine Franciscan Sisters in 1963.
The last day for students will be June 1. School officials are helping parents find other options for their children.